mouse anti chicken cd8a pe  (SouthernBiotech)

Journal: Poultry science

Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens.

doi: 10.1016/j.psj.2023.103098

Figure Lengend Snippet: Figure 2. Detection of the sorting purity of chicken CD8+, CD4+, CD4+ CD25 and CD4+ CD25+ T cells by flow cytometry. (A) Magnetic bead sorting of chicken CD8+ T cells A1: blank cells (total PBMC); A2: CD8+ T cells before cell sorting; A3: CD8+ T cells after cell sorting. (B) Mag- netic bead sorting of chicken CD4+ T cells B1: blank cells (total PBMC); B2: CD4+ T cells before cell sorting; B3: CD4+ T cells after cell sorting. (C) Magnetic bead sorting of chicken CD4+ CD25 T cells C1: blank cells (total PBMC); C2: CD4+ CD25 T cells before cell sorting; C3: CD4+

Article Snippet: The primary phycoerythrin (PE)-conjugated mouse anti-chicken CD8a or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 £ 108 cells.

Techniques: Cytometry, FACS

Journal: Poultry science

Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens.

doi: 10.1016/j.psj.2023.103098

Figure Lengend Snippet: Figure 5. Effects of rEmHPSP-2 and rEmHPSP-3 on the transcription of IFN-g and IL-2 in chicken CD8+ T cells (A) Effects of rEmHPSP-2 on the transcription of IFN-g and IL-2 in chicken CD8+ T cells. (B) Effects of rEmHPSP-3 on the transcription of IFN-g and IL-2 in chicken CD8+ T cells. The differences between groups were analyzed using Duncan method of 1-way ANOVA, where the same letter indicates no significant differ- ence (P > 0.05) and different letter indicates significant difference (P < 0.05).

Article Snippet: The primary phycoerythrin (PE)-conjugated mouse anti-chicken CD8a or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 £ 108 cells.

Techniques:

Journal: Virus Research

Article Title: Protection of laying chickens against the Canadian DMV/1639 infectious bronchitis virus infection through priming with heterologous live vaccine and boosting with heterologous or homologous inactivated vaccine

doi: 10.1016/j.virusres.2023.199281

Figure Lengend Snippet: Immunohistochemical detection of CD8+ T cells in trachea (a), lung (b), kidney (c), cecal tonsil (d), ovary (e), magnum (f), isthmus (g), and uterus (h) collected at 12 days following infection with the IBV/Ck/Can/17–036,989 strain. CD8+ T cells were stained with dark brown (indicated with black arrows). Scale bar = 50 µm.

Article Snippet: The tissue sections were then incubated overnight with mouse anti-chicken CD8a (Southern Biotech, Birmingham, AL, USA) diluted 1:100 in 2.5% goat serum at 4 °C.

Techniques: Immunohistochemical staining, Infection, Staining

Journal: Virus Research

Article Title: Protection of laying chickens against the Canadian DMV/1639 infectious bronchitis virus infection through priming with heterologous live vaccine and boosting with heterologous or homologous inactivated vaccine

doi: 10.1016/j.virusres.2023.199281

Figure Lengend Snippet: Image analysis of CD8+ T cells in trachea, lung, kidney, cecal tonsil, ovary, magnum, isthmus, and uterus collected at 12 days following infection with the IBV/Ck/Can/17–036,989 strain. The percentages of CD8+ T cells were compared between all experimental groups using 1-way ANOVA followed by Tukey's multiple comparisons test, and the error bars represent the SD. Significant differences are denoted by different letters above the bars ( P < 0.05).

Article Snippet: The tissue sections were then incubated overnight with mouse anti-chicken CD8a (Southern Biotech, Birmingham, AL, USA) diluted 1:100 in 2.5% goat serum at 4 °C.

Techniques: Infection

Journal: Food and Agricultural Immunology

Article Title: The effect of humic substances on gut microbiota and immune response of broilers

doi: 10.1080/09540105.2019.1707780

Figure Lengend Snippet: Figure 3. The effect of 0.8% of natural humic substances prepared from leonardite in feed on the per- centage of CD4+ CD8–, CD8+ CD4– and CD4+ CD8+ lymphocytes in broiler peripheral blood. Means with different superscripts are significantly different **p < .01.

Article Snippet: Tubes were centrifuged for 30 min at 600× g. MNL were recovered by aspirating white layer in plasma-LSM interphase, and the acquired sample was washed twice with PBS by centrifugation for 5 min at 250× g. Selected lymphocyte subpopulations were identified by direct staining with a subsequent combination of conjugated anti-chicken monoclonal antibodies (MoAb): CD4-FITC/ CD8a-R-PE/CD45-APC (Southern Biotech, USA).

Techniques: